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Position£ºProduct--> Product Seller |
¡¾Subject¡¿ £º |
Shotgun Library Construction Service |
¡¾Person to contact¡¿ £º |
Dianna |
¡¾Email¡¿ £º |
contact@cd-genomics.com |
¡¾Tel¡¿ £º |
1-631-626-9181 |
¡¾Date¡¿ £º |
2015-2-1 |
¡¾Details¡¿ £º |
CD Genomics offers highly flexible shotgun library construction services, including shotgun sequencing of the completed library. High quality random-sheared shotgun libraries of BAC clones and whole genomes are regularly produced at CD Genomics. Our shotgun library construction has been done with a variety of vectors such as plasmid, cosmid, fosmid, BAC, and YAC.
Construction Steps:
• Plasmid DNA purification and/or quality analysis of in-coming DNA
• Random shearing to produce a population of DNA fragments
• Ligation into a standard cloning vector and transformation
• Libraries submitted to customer as plates, glycerol stocks, or purified DNA
• QC by PCR or DNA sequencing
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