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                      | Position£ºProduct--> Product Seller |  
                      
                        
                          | ¡¾Subject¡¿ £º | Shotgun Library Construction Service |  
                          | ¡¾Person to contact¡¿ £º | Dianna |  
                          | ¡¾Email¡¿ £º | contact@cd-genomics.com |  
                          | ¡¾Tel¡¿ £º | 1-631-626-9181 |  
                          | ¡¾Date¡¿ £º | 2015-2-1 |  
                          | ¡¾Details¡¿ £º | CD Genomics offers highly flexible shotgun library construction services, including shotgun sequencing of the completed library. High quality random-sheared shotgun libraries of BAC clones and whole genomes are regularly produced at CD Genomics. Our shotgun library construction has been done with a variety of vectors such as plasmid, cosmid, fosmid, BAC, and YAC.
 
Construction Steps:
   • Plasmid DNA purification and/or quality analysis of in-coming DNA
  • Random shearing to produce a population of DNA fragments
  • Ligation into a standard cloning vector and transformation
  • Libraries submitted to customer as plates, glycerol stocks, or purified DNA
  • QC by PCR or DNA sequencing |  
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