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Position£ºProduct--> Product Seller
¡¾Subject¡¿ £º Full Length cDNA Library Construction service
¡¾Person to contact¡¿ £º Dianna
¡¾Email¡¿ £º contact@cd-genomics.com
¡¾Tel¡¿ £º 1-631-626-9181
¡¾Date¡¿ £º 2015-2-1
¡¾Details¡¿ £º Finding full-length target sequences is a key objective of many library screens. Our novel full-length cDNA synthesis procedure combines a proprietary process for reducing RNase H reverse transcriptase activity with unique techniques for mRNA isolation, 5´ cap full-length enrichment, and reduction of oligo(dT) priming. Together, these steps greatly enhance the likelihood of constructing a library with full length inserts, giving you better targets at which to aim. • Full Length Enriched cDNA Library Full length-enriched cDNA is usually produced using modified template-switching approach. The method combines cDNA synthesis and amplification and results in representative cDNA population enriched with full-length sequences even from small amount of starting materials. • Normalized Full Length Enriched cDNA Library The method combines cDNA synthesis and amplification and allows generating representative cDNA populations enriched with full-length sequences even from small amounts of starting material. After cDNA synthesis and amplification, double stranded cDNA is normalized using DSN-normalization method.

 
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