Lipase-catalyzed transesterification from renewable oils for biodiesel production has many advantages over chemical approaches though the latter has been put into the industrialization for biodiesel production. However, the low stability (poor operational life) and the high cost of the lipase have been thought to be the main hurdle to the industrialization of lipase-catalyzed biodiesel production. We have proposed a novel route and the operational life of the immobilized lipase could be improved over 50-fold than traditional enzymatic approaches. This novel route is thought to be very promising for the commercialization of biodiesel production since it would reduce the lipase cost dramatically by significantly improving the stability and the operational life of the lipase. The demonstrational test in a pilot plant with capacity of 100kg/d biodiesel was successful. A new plant with 20,000ton/year capacity has been constructed in Hunan, China and it was put into operation in Dec. 8, 2006. The results from the full scale operation are even better than in the pilot plant demonstration.