Strain Improvement
Random Mutation with Chemical and Physical Mutagens
High Throughput Screen (HTS):
Screen large number of mutants on agar plate with rational design strategy
Empirical screen and morphological Selection
Improve the titer
Reduce the impurities and side products
Reduce the broth viscosity
Optimize the growth and fermentation media
Shorten the cycle time
Strain stability
Culture preservation
Screening
A primary screening of 500 mutants per week in shake flask fermentation, followed by further confirmation.
Assay
HPLC units*12 with PhotoDiode Array
GC units*2
Capacity of 5000 samples per week
Media optimization
Optimize the level of media ingredients or alternative ingredients for improved mutants to achieve the maximum productivity and reduce the production cost.
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